Cell Biologist

About the job: Cell Biologist Cell Biologist needs 3+ years for MS - 5+ years for BS. Extensive hands-on experience with human iPSC culture, gene editing, and molecular biology techniques. Cell Biologist requires: Proficiency in gene editing and cell line development, including optimization of protocols, familiarity with flow-based sorting. Hands-on experience with cell delivery methods of gene editing tools. Excellent communication and interpersonal skills, with the ability to work effectively in a team-oriented environment. Strong organizational skills and diligent record keeping. Experience in pancreatic islet directed differentiation is highly desirable Hands-on experience in transitioning research-grade processes to GMP-grade is highly desirable. Mandatory weekend cell culture responsibilities on rotation basis. Cell Biologist duties: Enabling and advancing iPSC gene editing, cell line development and evaluation of differentiated cells. Routine iPSC cell culture, passaging and media making. Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening and delivery. Optimize and perform assays for on-/off-target analysis via PCR and NGS workflows. Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR. Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such as cell counts, viability, metabolite assessments. Assist scientists in the team to evaluate and design mitigation strategies to ensure genomic stability of the iPSC lines and drug substance. Collaborate cross-functionally to support transfer of R&D assays to GMP. Maintain accurate and detailed records of all experiments, synthesize results, prepare reports and present in meetings. Contribute to SOP development. Work seamlessly in executing workplans and meeting deadlines.