Research Assistant III

Posted 8 days ago

divihn integrationMorrisville (NC)

Biological Technicians Research and Development in the Physical, Engineering, and Life Sciences (except Nanotechnology and Biotechnology)

SENIORITY

Senior

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About the role

Research Assistant IIITesting & Lab Operations Perform assays: Execute neutralizing antibody (nAb) and total antibody assays on clinical (human) and non-clinical (animal/cell) samples. Cell Culture: Maintain cell lines required for live-virus or pseudovirus neutralization assays. Pipetting: Handle high-throughput manual pipetting. Reagent Prep: Formulate buffers, cell culture media, and assay reagents according to Standard Operating Procedures (SOPs).Quality Control & ComplianceCLIA Standards: Maintain strict adherence to CLIA, GCLP, and OSHA safety standards. Run Validations: Execute positive and negative controls for every assay batch to ensure data validity. Instrument Calibration: Clean, calibrate, and maintain microplate readers, incubators, and biosafety cabinets. Documentation: Complete precise batch records, sample logs, and equipment maintenance sheets. Sample & Data Management Sample Receipt: Accession, aliquot, and track clinical serum or plasma samples using a LIMS system. Inventory Control: Track expiration dates and stock levels of critical assay components like reference standards. Data Extraction: Collect raw data (e.g., fluorescence or absorbance) from plate readers. Preliminary Analysis: Calculate neutralization titers (like IC50/EC50) and total antibody concentrations for review by the Lab Director. Biosafety & Waste Biosafety Containment: Work safely within Biosafety Level 2 (BSL-2) or BSL-3 environments depending on the virus type. Waste Management: Neutralize and dispose of biohazardous liquid and solid waste according to regulations.

Requirements: 5+ years of experience. Minimum of a Bachelor's degree in related field. Master's in related field preferred. Experience with Microsoft Office suite. LIMS experience is required. Experience with LabVantage is preferred. Experience in a CLIA or regulated laboratory required. Experience working with Immunological assays. Experience with ELISA is strongly preferred.

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