Client is focused on the use and optimization of a semi-synthetic E. coli organism for the production of novel protein therapeutics. We harness the power of a revolutionary technology based on E. coli that has been engineered to utilize an expanded genetic alphabet composed of the four natural nucleotides and two additional, synthetic nucleotides. This technology allows the encoding and production of custom proteins with site-specific functionalities for the discovery and development of novel protein-based therapeutics. We are seeking an analytical scientist/mass spectrometrist to utilize and help develop novel analytical, proteomic, and metabolomic methods to optimize unnatural protein production as well as to further optimize the semi-synthetic organism.
Key Responsibilities:
Ability to serve as the analytical subject matter expert and work closely with colleagues across the company. Develop chromatography and mass spectrometry methods for characterizing the expressed proteins and assessing expression yields and the fidelity with which various non-canonical amino acids are incorporated. While use of the semi-synthetic organism for industrial scale therapeutic protein production has been validated, many aspects of the organism's physiology remain to be further optimized, and the successful applicant will work with colleagues focused on optimizing the organism's physiology. This will include both metabolomics and proteomics. Client places a premium on intra-company collaboration, and thus the desire to interact closely with all facets of the effort to develop and use the semi-synthetic organism for novel therapeutic protein production is a must. The successful applicant should also enjoy and excel at working collaboratively on fast paced projects with rapidly evolving goals.
Basic Qualifications:
PhD in a field related to analytical chemistry, proteomics, or metabolomics. Additional postdoctoral/industry experience is a benefit but not a requirement. Strong background in chromatographic separation, biophysical characterization (SPR, DLS, DSF, circular dichroism), and spectrophotometric techniques for proteins and protein conjugates. Familiarity with Agilent instrumentation (LC & Q-TOF LC/MS) and Open Lab/Chromeleon software is a benefit. Familiarity with the routine use of mass spectrometry for the characterization of proteins is essential. Familiarity with the use of mass spectrometry proteomics/metabolomics is beneficial, but not essential. Excellent written and oral communication skills; strong organizational skills and attention to detail.
